| Brampton United  | |
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| | | Web site: http://www.canadianlionsfc.com Founded: 2002 Stadium: Victoria Park Stadium Stadium Address: 20 Victoria Crescent, Brampton, ON L6T 1E4 League: Canadian Soccer League
History: Club relocated to Brampton and re-named from Canadian Lions for 2008 season. Former Brampton club, Brampton Stallions, was dissolved.
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| Brantford Galaxy  | |
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| Kingston  | |
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| London City  | |
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| Mississauga Eagles  | |
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| | | Founded: 2010 Nickname: Eagles Stadium: Hershey Centre Stadium Address: 5500 Rose Cherry Place, Mississauga, ON L4Z 4B6 League: Canadian Soccer League
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| Montreal Impact Academy  | |
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| Niagara United  | |
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| North York Astros  | |
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| SC Toronto  | |
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| | | Web site: http://www.sctoronto.ca Founded: 2001 Stadium: Lamport Stadium Stadium Address: 1151 King Street West, Toronto, ON M6K 1E9 League: Canadian Soccer League
History: Changed name from Toronto Supra to Portugal FC in 2008. Name changed again to SC Toronto prior to 2011 season.
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| Serbian White Eagles  | |
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| St. Catharines Wolves  | |
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| TFC Adademy  | |
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| Toronto Croatia  | |
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| Waterloo Region  | |
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| Windsor Stars  | |
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| | | Founded: 2004 Stadium: Windsor Stadium Stadium Address: College Ave, Wiindsor, ON N9B 1P6 League: Canadian Soccer League
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| York Region Shooters  | |
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